For molar concentration and other related product information, please refer to:    

CRISPR/Cas9 related enzyme proteins, antibodies, and other reagents

RNP Complex Formation and Target DNA binding  

1. Add the following components to a sterile, nuclease-free tube on ice:

2. Collect all components by a brief centrifugation. Incubate the reaction at 37 °C for 1 hour.

References:

1.  Deactivated CRISPR Associated Protein 9 for Minor-Allele Enrichment in Cell-Free DNA    Amin Aalipour et al. Clinical Chemistry 2018 Vol. 64, Issue 2 p307-p9162. Purified Cas9 Fusion Proteins for Advanced Genome Manipulation Jovan Mircetic et al. Small Methods 2017 1, 16000523. Disruptive non-disruptive applications of CRISPR/Cas9  Jonathan LSchmid-Burgk  Current Opinion in Biotechnology December 2017, Volume 48 Pages 203-2094. Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP  technology using recombinant CRISPR ribonucleoproteins Toshitsugu Fujita et al. Genes to Cells  Volume 21, Issue 4 April 2016  Pages 370–3775. High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding Evan A Boyle  et al. PNAS 2017 May, 114 (21) 5461-54666.CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells  Wulan Deng et al. PNAS 2015 vol. 112 | no. 38 p11870-11875

Related Products

NLS-Cas9-NLS     NLS-Cas9-EGFP                 NLS-Cas9(D10A)-2NLS nickase 

dCas9 (D10A & H840A)       NLS-dCas9-NLS        Biotinylated NLS-dCas9-NLS

dCas9-SBP       NLS-dCas9 -EGFP-NLS               LbCas12a (Cpf1) Nuclease

eSpCas9(1.1)-3NLS              SpCas9-HF1-3NLS           Cas9 ELISA kit

Cas9 Monoclonal Antibody    Cas9 monoclonal antibody, biotinylated

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